A “Business of Biotechnology” section in Chapter 1 describes biotech company organisation and structure, top biotechnology and pharmaceutical companies, and features updated data on the biotechnology industry worldwide. Concise overviews examine cutting-edge techniques and concepts including bioinformatics, proteomics, gene editing, and genomics, as well as detailed information on agricultural, medical, forensic, and regulatory issues that impact the biotechnology industry. Coverage of recent research and developments includes discussions of gene editing approaches like CRISPR, precision medicine, immunotherapies, biosimilar drugs, transgenic crops, 3D bioprinting of tissues and organs, the Human Microbiome Project, and the Cancer Atlas Genome Project. Tools of the Trade Boxes provide details on modern techniques and methods related to each chapter’s content and the biotech industry. Making a Difference inspires and engages students by discussing how real people, real companies, and real organisations are putting biotech to use to improve the quality of life. Making a Difference appears at the end of each chapter and provides short case studies that contain an example of the chapter’s content and how it can be applied in the real world. A revised Chapter 12 discusses international regulatory authorities that oversee biotechnology regulations. Forecasting the Future begins each chapter and highlights biotechnology-driven questions that have yet to be answered, are in the process of being researched, or are topics/research/policy that are under development and will have an impact on our future. This feature demonstrates to students that biotechnology is a dynamic field, encompassing a broad range of career options, and that there are still many problems and questions that they can get involved in solving and answering.