Bananas and plantains (Musa spp.) are amongst the most important tropical and subtropical food crops in the world. In order to reduce the impact of biotic and abiotic factors on banana cultivation, it is important to allow for a genetic enrichment of the currently cultivated genepool. Crop Wild Relatives play an important role in modern plant breeding as they contain important traits for agriculture. Their conservation is therefore essential for future sustainable agriculture and food security. This chapter focusses on the different aspect and techniques that are required to establish seed collections of wild bananas. This covers physiology and morphology of the banana seeds, difficulties encountered during their germination and putative solutions, possibilities for medium and long term storage and population genetics.